Technologies

Our state-of-the-art portfolio of biophysical technologies enables us to provide you with a complete and comprehensive picture of your specific question: From affinity and stability to kinetics and thermodynamics.

KD | EC50 | Tm | ΔTm | Tagg | ΔTagg | kon | koff | n | ΔH | ΔG | ΔS | rh | PDI | Tsize | kD

Automated MST

APPLICATION AREAS

  • Drug discovery
  • Fragment screening
  • Compound screening
  • Protein interaction analysis

PARAMETERS

  • Single-dose binding
  • Steady-state affinity (KD)

NANOTEMPER CERTIFIED SERVICE PROVIDER

Dianthus

APPLICATION AREAS

  • Drug discovery
  • Fragment screening
  • Compound screening

PARAMETERS

  • Single-dose binding
  • Steady-state affinity (KD)

NANOTEMPER CERTIFIED SERVICE PROVIDER

WAVEsystem

APPLICATION AREAS

  • Drug discovery
  • Fragment screening
  • Compound screening
  • Off-rate ranking
  • Biologics development

PARAMETERS

  • Binding kinetics (kon, ka, koff, kd)
  • Steady-state affinity (KD)
  • Enthalpy (ΔH)

CREOPTIX CERTIFIED SERVICE PROVIDER

MicroScale Thermophoresis (MST Service) is a biophysical technique that measures the strength of interaction (affinity) between two molecules by detecting variations in fluorescence signal as a result of an IR-laser induced temperature change. The range of the variation in the fluorescence signal correlates with the binding of a ligand to the fluorescent target. By that it allows for quantitative analysis of molecular interactions in solution on the microliter scale with high sensitivity. This page gives an overview of the MicroScale Thermophoresis (MST) method. You can find information on the core technology and typical applications of MST. Also detailed information on labeling and fluorescence requirements and the central advantages of the method are given.

MST

APPLICATION AREAS

  • Small-scale interaction analysis
  • Assay development
  • Aptamer binding
  • Protein-protein interaction analysis
  • Competition assays

PARAMETERS

  • Single-dose binding
  • Steady-state affinity (KD)

NANOTEMPER CERTIFIED SERVICE PROVIDER

nanoDSF

APPLICATION AREAS

  • Protein stability and unfolding analysis
  • Buffer screening and optimization
  • Protein aggregation analysis
  • Pre- and formulation development
  • Ligand binding (thermal shift)

PARAMETERS

  • Protein unfolding (Tm, Tonset, Tturbidity, kd)
  • Binding (Tm-diff, EC50)

NANOTEMPER CERTIFIED SERVICE PROVIDER

DLS

APPLICATION AREAS

  • Protein quality control
  • Particle size determination
  • Solutio homogeneity
  • Protein aggregation analysis
  • Pre- and formulation development
  • Buffer screening and optimization

PARAMETERS

  • Paticle parameters (rh, PDI)
  • Aggregation analysis (Tsize, Tscattering)

NANOTEMPER CERTIFIED SERVICE PROVIDER

BLI

APPLICATION AREAS

  • Biologics development
  • Antibody-antigen interaction analysis
  • Off-rate ranking

PARAMETERS

  • Binding kinetics (kon, ka, koff, kd)
  • Steady-state affinity (KD)
  • Enthalpy (ΔH)

ITC

APPLICATION AREAS

  • Thermodynamic interaction analysis
  • Drug discovery
  • Compound and fragment binding
  • Protein-protein interaction analysis

PARAMETERS

  • Steady-state affinity (KD)
  • Binding stoichiometry (n)
  • Binding thermodynamics (ΔH, ΔS, ΔG)

LabCyte Echo

APPLICATION AREAS

  • Compound spotting/printing
  • Fragment spotting/printing
  • Contact-free, high-precision dilution series
  • Industry-standard
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