Isothermal Titration Calorimetry – Advantages

Isothermal Titration Calorimetry – Advantages

In general, ITC is the go-to method for determining high-quality thermodynamic parameters of molecular interactions. Moreover, it provides a direct, label-free, and in-solution approach.

No label required
→ close to native molecular state/conformation


No immobilization required
→ No influence on molecular conformation/stability


Wide temperature range
→ 10 to 80 °C


Wide range of possible molecules and ligands
→ No limitation in molecular weight


Wide range of possible buffers and solvents
→ Only few additives may cause background heat signals


Relatively low sample consumption for ITC standards
→ Only 280 µL of macromolecule (at around 20 µM)
→ Only 40 µL of ligand (at around 200 µM)


Complete thermodynamic characterization in one experiment
→ Enthalpy, entropy, and free enthalpy of binding


Determination of binding characteristics
→ Dissociations constant and reaction stoichiometry


Wide range of binding affinity measurable
→ Millimolar to nanomolar dissociation constants