Epigenetic analytical services

We analyze your epigenetic interaction using our innovative MST platform

Epigenetic regulation is based on specific molecular interactions between epigenetic reader, writer and eraser molecules and chromatin. Binding parameters of these interactions such as binding affinities, stoichiometries and thermodynamics are essential for the understanding of the establishment and maintenance of epigenetic networks. Our rapid and precise MicroScale Thermophoresis (MST) platform is optimized to characterize your epigenetic interactions in solution at microliter scale, requiring low concentrations of the potential interactors.

We analyze the interaction between your epigentic effector protein and …:

…DNA binding sites

  • We characterize interactions between transcription factors and their binding sites

We characterize the interaction of your transcription factor to their binding sites using our MicroScale Thermophoresis plattform.

MicroScale Thermophoresis - transcription factor binding to DNA binding site

Pham et. al., NAR, 2013: Replot of MicroScale Thermophoresis of the PU.1 transcription factor – DNA binding site interaction

 

…Core histones

  • We characterize interactions between epigenetic effector proteins and core histones

We characterize the interaction of your epigenetic effector protein of interest and core histones using our MicroScale Thermophoresis plattform.

MicroScale Thermophoresis - DF31 binding to core histones

Schubert et al. Mol. Cell, 2012: MicroScale Thermophoresis analysis of the Df31 protein – core histone interaction.

…Histone tails

  • We characterize interactions between epigenetic effector proteins and histone tails

We characterize the interaction of your epigenetic effector protein of interest and histone tails using our MicroScale Thermophoresis plattform. The binding of your protein to different histone tails with different modifications is analyzed.
Bromo 3 all modifications
Josling et al., Host Cell & Microbe, 2015: MicroScale Thermophoresis analysis of the BDP1 protein – histone tail interactions.

…Chromatin templates

  • We characterize interactions between epigenetic effector proteins and chromatin templates

We characterize the interaction of your epigenetic effector protein of interest and a chromatin template (mononucleosomes, dinucleosomes or arrays) using our MicroScale Thermophoresis plattform.
MicroScale Thermophoresis analysis of DF31 - mononucleosome interaction

Schubert et al. AIMS Biophysics, 2015: MicroScale Thermophoresis analysis of the Df31 protein – mononucleosome interaction.

 

…Nucleic acids

  • We characterize interactions between epigenetic effector proteins and nucleic acids

We characterize the interaction of your epigenetic effector protein of interest and nucleid acids using our MicroScale Thermophoresis plattform.

MicroScale Thermophoresis - DF31 binding to nucleic acids

Schubert et al. Mol. Cell, 2012: MicroScale Thermophoresis analysis of the Df31 protein – nucleic acid interaction.

…Drug candidates

  • We characterize interactions between epigenetic effector proteins and potential drugs

We characterize the interaction of your epigenetic effector protein of interest and potential drug candidates using our MicroScale Thermophoresis plattform

MicroScale Thermophoresis analysis of BDP1 - drug interactions

Schubert et al. AIMS Biophysics, 2015: MicroScale Thermophoresis analysis of the Df31 protein – mononucleosome interaction.