Biolayer Interferometry – Advantages

Biolayer Interferometry – Advantages

In general, ITC is the go-to method for determining high-quality thermodynamic parameters of molecular interactions. Moreover, it provides a direct, label-free, and in-solution approach.

Label-free detection
→ No influence of fluorescent or other dyes

 

Complete kinetic characterization of a molecular interaction
→ Gives association and dissociation rate constants

 

Wide range of binding affinity measurable
→ Millimolar to nanomolar dissociation constants

 

Wide range of possible buffers and solvents
→ For example also high concentrations of glycerol and DMSO

 

Possibility to measure in crude biological liquids or samples
→ For example serum or cell lysate

 

Wide temperature range
→ 4 to 40 °C

 

Wide range of possible molecules and ligands
→ small 150 Da compounds, peptides, DNA, RNA, proteins, up to bacteria, viruses, and cells

 

Wide range of binding affinity measurable
→ Millimolar to nanomolar dissociation constants