Publications

2017

Aptatope mapping of the binding site of a progesterone aptamer on the steroid ring structure

Skouridou V, Schubert T, Bashammakh AS, El-Shahawi MS, Alyoubi AO, O’Sullivan CK.
Anal Biochem. 2017 May 10

The Composition of the Arabidopsis RNA Polymerase II Transcript Elongation Complex Reveals the Interplay between Elongation and mRNA Processing Factors

Antosz W, Pfab A, Ehrnsberger HF, Holzinger P, Köllen K, Mortensen SA, Bruckmann A, Schubert T, Längst G, Griesenbeck J, Schubert V, Grasser M, Grasser KD
Plant Cell. 2017 Apr

The Arabidopsis THO/TREX component TEX1 functionally interacts with MOS11 and modulates mRNA export and alternative splicing events

Sørensen BB, Ehrnsberger HF, Esposito S, Pfab A, Bruckmann A, Hauptmann J, Meister G, Merkl R, Schubert T, Längst G, Melzer M, Grasser M, Grasser KD.
Plant Mol Biol. 2017 Feb

Mapping the Binding Site of an Aptamer on ATP Using MicroScale Thermophoresis

Entzian C, Schubert T.
J Vis Exp. 2017 Jan 7

2016

The characterization and validation of 17β-estradiol binding aptamers

Svobodová M, Skouridou V, Botero ML, Jauset-Rubio M, Schubert T, Bashammakh AS, El-Shahawi MS, Alyoubi AO, O’Sullivan CK.,
J Steroid Biochem Mol Biol, 2016

ß-Conglutin dual aptamers binding distinct aptatopes

Miriam Jauset Rubio, Markéta Svobodová, Teresa Mairal, Thomas Schubert, Stefan Künne, Günter Mayer, Ciara K. O’Sullivan, Anal
Bioanal Chem, 2016

Aptamer Binding Studies Using MicroScale Thermophoresis

Dennis Breitsprecher, Nina Schlinck, David Witte, Stefan Duhr, Philipp Baaske, and Thomas Schubert; Chapter 8, Nucleic Acid Aptamers,
Springer Protocols; Methods in Molecular Biology, Humana Press, 2016

2015

Studying small molecule–aptamer interactions using MicroScale Thermophoresis (MST)

Clemens Entzian, Thomas Schubert, 2bind GmbH, Josef Engert Straße 13, 93053 Regensburg, Germany,
Methods, 2015 Aug 31

Synonymous variants in HTRA1 implicated in AMD susceptibility impair its capacity to regulate TGF-β signaling

Friedrich U1, Datta S1, Schubert T2, Plössl K1, Schneider M3, Felix G1, Fuchshofer R3, Tiefenbach KJ4, Längst G5, Weber BH6; 1 Institute of Human Genetics, University of Regensburg, 2 Department of Biochemistry, University of Regensburg, Germany 2bind GmbH, 3 Institute of Human Anatomy and Embryology, University of Regensburg, 4 Institute of Biophysics and Physical Biochemistry, University of Regensburg, 5 Department of Biochemistry, University of Regensburg, 6 Institute of Human Genetics, University of Regensburg, Franz-Josef-Strauss-Allee 11, 93053 Regensburg, Germany; Hum Mol Genet, 2015 Aug 26

Review: Studying epigenetic interactions using MicroScale Thermophoresis (MST)

Thomas Schubert 1, * and Gernot Längst 2 1 2bind GmbH, Josef Engertstraße 13, 93053 Regensburg, Germany 2 Biochemie III, Universität Regensburg, Universitätstraße 31, 93053 Regensburg, Germany AIMS Biophysics, 2015 Aug 21

In vitro Selection and Interaction Studies of a DNA Aptamer Targeting Protein A

Regina Stoltenburg 1 ,Thomas Schubert 2, Beate Strehlitz 3 UFZ—Helmholtz Centre for Environmental Research, Department of Soil Ecology, Halle, Germany; 2bind GmbH, Regensburg, Germany; UFZ—Helmholtz Centre for Environmental Research, Department Environmental and Biotechnology Centre, Leipzig, PLOS ONE, 2015 July 29

the official link to the online-presentation of the PLOS ONE paper :

https://globalmedicaldiscovery.com/key-medical-diagnostics-articles/in-vitro-selection-interaction-studies-dna-aptamer-targeting-protein-a/

A Plasmodium Falciparum Bromodomain Protein Regulates Invasion Gene Expression

Gabrielle A. Josling, Michaela Petter, Sophie C. Oehring, Archna P. Gupta, Olivier Dietz, Danny W. Wilson, Thomas Schubert, Gernot Längst, Paul R. Gilson, Brendan S. Crabb, Suzette Moes, Paul Jenoe, Shu Wei Lim, Graham V. Brown, Zbynek Bozdech, Till S. Voss, Michael F. Duffy 1 1 Department of Medicine Royal Melbourne Hospital, The Peter Doherty Institute, The University of Melbourne, 792 Elizabeth Street, Melbourne, Victoria 3000, Australia Germany Cell Host and Microbe, 2015 June 10

2014

Genes Dev. 2014 April 1.

The NHL domain of BRAT is an RNA-binding domain that directly contacts the hunchback mRNA for regulation

Inga Loedige, Mathias Stotz, Saadia Qamar, Katharina Kramer, Janosch Hennig, Thomas Schubert, Patrick Löffler, Gernot Längst, Rainer Merkl, Henning Urlaub, and Gunter Meister Department of Biochemistry I, Biochemistry Center Regensburg, University of Regensburg, Universitätsstr. 31, D-93053 Regensburg, Germany. Department of Biochemistry III, Biochemistry Center Regensburg, University of Regensburg, Universitätsstr. 31, D-93053 Regensburg, Germany. 2014 Genes Dev. BioCentury, February 2014.

Read about 2bind in the emerging company profile – 2bind GmbH of BioCentury

 

2013

Nucleic Acids Res. 2013 May 8.

Mechanisms of in vivo binding site selection of the hematopoietic master transcription factor PU.1.

Pham TH, Minderjahn J, Schmidl C, Hoffmeister H, Schmidhofer S, Chen W, Längst G, Benner C, Rehli M. Department of Internal Medicine III, University Hospital Regensburg, F.-J.-Strauss Allee 11, D-93042 Regensburg, Germany, Department of Biochemistry III, University of Regensburg, Universitätsstrasse 31, D-93053 Regensburg, Germany,   Nucleic Acids Res. 2013 Apr 10. [Epub ahead of print] NAR, 2013 may 1

Large-scale organization of ribosomal DNA chromatin is regulated by Tip5.

Zillner K, Filarsky M, Rachow K, Weinberger M, Längst G, Németh A. Department of Biochemistry III, Biochemistry Center Regensburg, University of Regensburg, Universitätsstr. 31, D-93053 Regensburg, Germany.     PLoS One, Epub 2013 Mar 26.

Arabidopsis DEAD-Box RNA Helicase UAP56 Interacts with Both RNA and DNA as well as with mRNA Export Factors.

Kammel C, Thomaier M, Sørensen BB, Schubert T, Längst G, Grasser M, Grasser KD. Department of Cell Biology and Plant Biochemistry, Biochemie-Zentrum Regensburg, University of Regensburg, Regensburg, Germany. Department of Biochemistry III, Biochemistry Center Regensburg, University of Regensburg, Universitätsstr. 31, D-93053 Regensburg, Germany.

2012

Mol Cell. 2012 Nov 9

Df31 protein and snoRNAs maintain accessible higher-order structures of chromatin.

Schubert T, Pusch MC, Diermeier S, Benes V, Kremmer E, Imhof A, Längst G. Institut für Biochemie III, Universität Regensburg, Universitätsstrasse 31. 93053 Regensburg, Germany.

Methods Mol Biol. 2012;815:241-52. doi: 10.1007/978-1-61779-424-7_18.

Microscale thermophoresis as a sensitive method to quantify protein: nucleic acid interactions in solution.

Zillner K, Jerabek-Willemsen M, Duhr S, Braun D, Längst G, Baaske P. Biochemistry III, Universität Regensburg, Regensburg, Germany.