• From Stability to Affinity


    nanoDSF and MicroScale Thermophoresis Services…
  • MicroScale Thermophoresis


    label-free and fluorescent binding assays in solution …
  • nanoDSF


    highest resolution of melting temperatures…
  • MicroScale Thermophoresis


    binding parameters with great precision…
  • nanoDSF


    fast stability information at low sample consumption…

2bind – The provider for nanoDSF and MicroScale Thermophoresis services

nanoDSF to study protein stabilty

We recently included the innovative nanoDSF technology, which is used to characterize proteins in terms of stability, in our service portfolio. This fast and flexible method allows for quantification of melting temperatures of proteins in a labelfree and buffer/detergent independent manner. The nanoDSF is able to analyze protein solution with broad concentration range (200mg/ml to 5µ g/ml) offering important information on formulation. Furthermore, nanoDSF enables us to precisely study previously challenging target proteins such as membrane proteins. Besides nanoDSF assays to optimize buffer conditions, we offer screening assays for optimal detergents, screening assays for the optimal formulation and screening assays for binding partners. In addition, we use the nanoDSF to find the optimal conditions for interaction analyses by MicroScale Thermophoresis (MST). This way we combine the synergies between these invocative technologies.

MicroScale Thermophoresis to characterize molecular interactions of any kind (MST)

MicroScale Thermophoresis (MST) is a powerful tool used to analyze molecular interactions. This technology allows our experts to study any kind of molecular interaction in solution, with free choice of buffer, allowing close to native measurements in sera and lysates. Our semi-automated MicroScale Thermophoresis platform is optimized in terms of speed, sample consumption, and cost-efficiency. Meanwhile, several integrated quality controls enables us to easily identify sticking and aggregation effects and to directly improving the reaction conditions, ensuring high quality data. Besides the low sample consumption our customers benefit from the broad application range of our MicroScale Thermophoresis services such as screening assays, binding assays, competition assays, stoichiometry assays, buffer optimization assays, or assays with multiple binding partners, which all provide a high information content. Therefore, our MicroScale Thermophoresis Services are perfectly suited for the identification and characterization of molecular interactions. Our customers and collaborators mainly work in the fields of drug development, aptamer generation, and antibody discovery. For more information on the MST technology please read 2bind MicroScale Thermophoresis information booklet. For more information on our MST services please read the flyer 2bind – MicroScale Thermophoresis services Please contact us if you are interested in our Services. Check out our Microscale Thermophoresis analyses mapping the precise binding site of an aptamer directed against AMP/ATP: 2bind-AMP-mapping Read more about the MicroScale Thermophoresis aptamer-small molecule study BMBF_RGB_Gef_L_e